PA5-29163

antibody from Invitrogen Antibodies

Targeting: CDC45 CDC45L, CDC45L2

Antibody data

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Validation data

Product number: PA5-29163 - Provider product page
Provider: Invitrogen Antibodies
Product name: Cdc45L Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: Jurkat, Raji, NCI-H929, A549, HeLa, AGS. Predicted reactivity: Mouse (93%), Rat (92%), Bovine (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.73 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

CDC45 protein structure - PA5-29163 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Cdc45L was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Cdc45L Polyclonal Antibody (Product # PA5-29163) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Cdc45L Polyclonal Antibody (Product # PA5-29163). Sample (30 µg of whole cell lysate). Lane A: HeLa . 7.5% SDS PAGE. Cdc45L Polyclonal Antibody (Product # PA5-29163) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Cdc45L was achieved by transfecting K-562 with Cdc45L specific siRNAs (Silencer® select Product # S15830, S15829). Western blot analysis (Fig. a) was performed using Whole Cell Extract (1% SDS) from the Cdc45L knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Cdc45L Polyclonal Antibody (Product # PA5-29163, 1:2000 dilution ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:2000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Cdc45L.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Cdc45L Polyclonal Antibody (Product # PA5-29163) and a 60 kDa band corresponding to Cdc45L was observed across cell lines and tissue tested along with an uncharacterized (*) band at ~110 kDa. Whole Cell Extract (1% SDS) (40 µg lysate) of K-562 (Lane 1), T98G (Lane 2), Jurkat (Lane 3), Rat Testis (Lane 4), Rat Liver (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).