Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- PA5-47801 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LCTL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Lactase-like Protein/LCTL is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of A549 human lung carcinoma cell line and A431 human epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-human Lactase-like Protein/LCTL Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47801) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Lactase-like Protein/LCTL at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LCTL in A549 human lung carcinoma cell line and A431 human epithelial carcinoma cell line. Samples were incubated in LCTL polyclonal antibody (Product # PA5-47801) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Lactase-like Protein/LCTL at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions.