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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- BS4702 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- LAT (phospho Y191) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide derived from human LAT (Isoform Short) around the phosphorylation site of Tyrosine 191.
- Description
- LAT, a transmembrane adaptor protein expressed in T, NK and mast cells, is an important mediator for T cell receptor (TCR) signaling. Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets. The phosphorylation of LAT at Tyr171 and 191 enables the binding of Grb2, Gads/SLP-76, PLCgamma1 and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of p-LAT (Y191) pAb at 1:500 dilutionLane1:Hela cell lysate treated with PMA(100nM,15mins)Lane2:Raw264.7 cell lysate treated with PMA(100nM,15mins)Lane3:PC12 cell lysate treated with PMA(100nM,15mins)
