Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB6334 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal LAT Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Phospho-LAT (Y161) in Western blots.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human Phospho-LAT (Y161) by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with Pervanadate (PV) for 5 minutes. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human Phospho-LAT (Y161) Monoclonal Antibody (Catalog # MAB6334) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-LAT (Y161) at approximately 40 kDa (as indicated). For additional reference, the membrane was stripped and reprobed with 1 µg/mL of Sheep Anti-Human LAT Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # AF6208). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.