Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- 14-9967-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LAT Monoclonal Antibody (LAT.10-17 (10-17)), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This LAT.10-17 (10-17) monoclonal antibody reacts with human Linker for Activation of T cells (LAT), which is essential for T cell receptor (TCR)-mediated signal transduction. Characterized as an integral transmembrane protein, this ~37-kDa molecule does not possess a true extracellular domain. Upon TCR/CD3 engagement, LAT localizes to lipid rafts and is tyrosine phosphorylated by ZAP-70, leading to the recruitment and activation of other downstream signaling molecules such as Grb-2, PLCgamma, and PI3K. This adapter molecule has been shown to be required for T cell activation, as well as thymocyte and T regulatory cell development. In humans, LAT is expressed on peripheral blood lymphocytes, lymph nodes, and tonsil. Studies in mice have shown that LAT is expressed in thymocytes, T cells, mast cells, natural killer cells, megakaryocytes, platelets, and early B cells. This antibody does not crossreact with the mouse antigen. Applications Reported: This LAT.10-17 (10-17) antibody has been reported for use in immunoblotting (WB) and immunoprecipitation (IP). Applications Tested: LAT.10-17 (10-17) was tested by western blot and immunoprecipitation on lysates prepared from human peripheral blood cells, Jurkat cells, and C57BL/6 splenocytes. This antibody can be used at 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- LAT.10-17 (10-17)
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references T-cell receptor signaling to integrins.
LAT-mediated signaling in CD4+CD25+ regulatory T cell development.
Role of the LAT adaptor in T-cell development and Th2 differentiation.
Burbach BJ, Medeiros RB, Mueller KL, Shimizu Y
Immunological reviews 2007 Aug;218:65-81
Immunological reviews 2007 Aug;218:65-81
LAT-mediated signaling in CD4+CD25+ regulatory T cell development.
Koonpaew S, Shen S, Flowers L, Zhang W
The Journal of experimental medicine 2006 Jan 23;203(1):119-29
The Journal of experimental medicine 2006 Jan 23;203(1):119-29
Role of the LAT adaptor in T-cell development and Th2 differentiation.
Malissen B, Aguado E, Malissen M
Advances in immunology 2005;87:1-25
Advances in immunology 2005;87:1-25
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoblotting of 10e6 normal peripheral human blood cells (lane 1), Jurkat (lane 2), and C57BL/6 splenocytes (lane 3) with 5 µg/mL Anti-Human LAT Purified. Bands were visualized using Anti-Mouse IgG HRP.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-LAT Monoclonal Antibody (LAT.10-17 (10-17)), eBioscience™ (Product # 14-9967-82) and 34kDa, 28kDa, 27kDa and 24kDa bands corresponding to LAT was observed in T cell line, Jurkat, and Natural Killer cells, NK-92, but not in B-cells (Raji and Daudi). Whole Cell Extracts (30 µg lysate) of Jurkat (Lane 1), Raji (Lane 2), Daudi (Lane 3) and NK-92 (Lane 4) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1.5ug/mL dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:8000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Linker for T cell activation (LAT) is preferentially expressed in higher levels in T-cells and Natural Killer cells for its development and proliferation. Also, proteolytically cleaved products, reported at several molecular weights >13kDa, are detected in Jurkat and NK-cells in comparison to the B-cell lines tested. [doi:10.3389/fimmu.2018.00115, 10.1042/BJ20121135]