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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- ABIN1504608 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Linker For Activation of T Cells (LAT) antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Protein A Chromatography
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.2 mg
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- Western Blot: Jurkat cells were incubated without (Lanes 1,3) or with (Lanes 2,4) monoclonal antibody that binds Jurkat T-cell receptors. Lysates were probed with anti-phospho-LAT (Tyr191) (2?g/ml) (Lanes 1,2), or anti-LAT (1 ?g/ml) (Lanes 3,4).
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunoblot Analysis (Representative lot data): Unstimulated and stimulated Jurkat cells (lanes 1 and 2) were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-LAT (Tyr191) (1.0 μg/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phospho-LAT. Arrow indicates anti-phospho LAT (Tyr191).
