Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-99093 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RFNG Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 3.09 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RFNG using a RFNG Polyclonal antibody (Product # PA5-99093). Positive WB detected in: HepG2 whole cell lysate, 293 whole cell lysate, PC3 whole cell lysate, Rat brain tissue. All lanes: RFNG antibody at 1:2000. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 37 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RFNG using a RFNG Polyclonal antibody (Product # PA5-99093). Positive WB detected in: HepG2 whole cell lysate, 293 whole cell lysate, PC3 whole cell lysate, Rat brain tissue. All lanes: RFNG antibody at 1:2000. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 37 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RFNG in paraffin embedded human adrenal gland tissue using a RFNG polyclonal antibody (Product # PA5-99093) at a dilution of 1:500. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.