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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA325124 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal SMAD3-S208 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal SMAD3-S208 Antibody
- Reactivity
- Porcine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- SMAD3
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- SMAD3 Antibody (S208) (Cat. #TA325124) western blot analysis in HepG2 and mouse C2C12 cell line lysates (35ug/lane).This demonstrates the SMAD3 antibody detected the SMAD3 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of SMAD3-S208 Antibody(Cat#TA325124) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- SMAD3-S208 Antibody (cat. #TA325124) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SMAD3-S208 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- SMAD3-S208 Antibody (Cat. #TA325124) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
