Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A305-593A-T - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AAGAB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 10 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human AAGAB by western blot. Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, Jurkat, U2OS, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AAGAB antibody ((Product # A305-593A-T lot 2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human AAGAB by western blot. Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, Jurkat, U2OS, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AAGAB antibody ((Product # A305-593A-T lot 2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse AAGAB by western blot. Samples: Whole cell lysate (10 µg) from BeWo, AlphaTC1 Clone 9, and A20 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AAGAB antibody (Product # A305-593A-T lot 2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human AAGAB by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-AAGAB antibody A305-593A-M (lot A305-593A-M-1) used for IP at 50 µl per reaction. AAGAB was also immunoprecipitated by rabbit anti-AAGAB antibody BL22311. For blotting immunoprecipitated AAGAB, A305-593A-M was used at 1:400. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse AAGAB by western blot. Samples: Whole cell lysate (15 µg) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AAGAB antibody A305-593A-M (lot A305-593A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human AAGAB by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-AAGAB antibody (Product # A305-593A-T; lot 2) used for IP at 20 µL per reaction. AAGAB was also immunoprecipitated by a previous lot of this antibody (Product # A305-593A-M lot 1) and rabbit anti-AAGAB antibody BL22311. For blotting immunoprecipitated AAGAB, Product # A305-593A-T was used at 1:1000 dilution. Detection: Chemiluminescence with an exposure time of 75 seconds.
