PA5-114392

antibody from Invitrogen Antibodies

Targeting: PHF21A BHC80, BM-006, KIAA1696

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-114392

Provider

Invitrogen Antibodies

Product name

PHF21A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL. Positive Control - WB: human A549 whole cell, rat brain tissue, mouse brain tissue. IHC: human Ovarian cancer tissue, human oesophagus squama cancer tissue, human tonsil tissue, human endometrial carcinoma tissue, human gastric cancer tissue, human intestinal cancer tissue, human lung cancer tissue, human placenta tissue, human sarcoma tissue, human glioma tissue. Flow: HeLa cell, SiHa cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

500 µg/mL

Storage

-20°C

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of PHF21A in human esophagus squama cancer tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in PHF21A polyclonal antibody (Product # PA5-114392) at a dilution of 1 µg/mL (overnight, 4°C), followed by Biotin conjugated goat anti-rabbit IgG (30 min, 37°C) and DAPI at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of PHF21A in human intestinal cancer tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in PHF21A polyclonal antibody (Product # PA5-114392) at a dilution of 1 µg/mL (overnight, 4°C), followed by Biotin conjugated goat anti-rabbit IgG (30 min, 37°C) and DAPI at a dilution of 1:100.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PHF21A in paraffin-embedded human tonsil tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in PHF21A polyclonal antibody (Product # PA5-114392) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PHF21A in paraffin-embedded human sarcoma tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in PHF21A polyclonal antibody (Product # PA5-114392) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PHF21A in paraffin-embedded human Ovarian cancer tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in PHF21A polyclonal antibody (Product # PA5-114392) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PHF21A in paraffin-embedded human placenta tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in PHF21A polyclonal antibody (Product # PA5-114392) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PHF21A in paraffin-embedded human lung cancer tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in PHF21A polyclonal antibody (Product # PA5-114392) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PHF21A in paraffin-embedded human intestinal cancer tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in PHF21A polyclonal antibody (Product # PA5-114392) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PHF21A in paraffin-embedded human gastric cancer tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in PHF21A polyclonal antibody (Product # PA5-114392) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PHF21A in paraffin-embedded human endometrial carcinoma tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in PHF21A polyclonal antibody (Product # PA5-114392) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of PHF21A in paraffin-embedded human intestinal cancer tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in PHF21A polyclonal antibody (Product # PA5-114392) at a dilution of 1 µg/mL (overnight, 4°C), followed by Biotin conjugated goat anti-rabbit IgG (30 min, 37°C) and DAPI at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PHF21A in paraffin-embedded human esophagus squama cancer tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in PHF21A polyclonal antibody (Product # PA5-114392) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PHF21A in paraffin-embedded human glioma tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in PHF21A polyclonal antibody (Product # PA5-114392) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of PHF21A in paraffin-embedded human esophagus squama cancer tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in PHF21A polyclonal antibody (Product # PA5-114392) at a dilution of 1 µg/mL (overnight, 4°C), followed by Biotin conjugated goat anti-rabbit IgG (30 min, 37°C) and DAPI at a dilution of 1:100.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of PHF21A in SiHa cells. Samples were blocked with 10% normal goat serum and incubated in PHF21A polyclonal antibody (Product # PA5-114392) at a dilution of 1 µg/1x10^6 cells (30 min, 20°C), followed by DyLight 488 conjugated goat anti-rabbit IgG (30 min, 20°C) with a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C). Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) and unlabeled sample (Red line).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of PHF21A in HeLA cells. Samples were blocked with 10% normal goat serum and incubated in PHF21A polyclonal antibody (Product # PA5-114392) at a dilution of 1 µg/1x10^6 cells (30 min, 20°C), followed by DyLight 488 conjugated goat anti-rabbit IgG (30 min, 20°C) with a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C). Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) and unlabeled sample (Red line).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of PHF21A in HeLA cells. Samples were blocked with 10% normal goat serum and incubated in PHF21A polyclonal antibody (Product # PA5-114392) at a dilution of 1 µg/1x10^6 cells (30 min, 20°C), followed by DyLight 488 conjugated goat anti-rabbit IgG (30 min, 20°C) with a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C). Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) and unlabeled sample (Red line).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of PHF21A in SiHa cells. Samples were blocked with 10% normal goat serum and incubated in PHF21A polyclonal antibody (Product # PA5-114392) at a dilution of 1 µg/1x10^6 cells (30 min, 20°C), followed by DyLight 488 conjugated goat anti-rabbit IgG (30 min, 20°C) with a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C). Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) and unlabeled sample (Red line).