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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- ANT-032-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LINGO-1 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate: - 1. Anti-LINGO-1 (extracellular) Antibody (#ANT-032), (1:500). 2. Anti-LINGO-1 (extracellular) Antibody , preincubated with LINGO-1 (extracellular) Blocking Peptide (#BLP-NT032).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4)brain membranes: - 1,2. Anti-LINGO-1 (extracellular) Antibody (#ANT-032), (1:200).3,4. Anti-LINGO-1 (extracellular) Antibody , preincubated with LINGO-1 (extracellular) Blocking Peptide (#BLP-NT032).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of LINGO-1 in rat brain and spinal cord - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-LINGO-1 (extracellular) Antibody (#ANT-032), (1:400), followed by donkey- Anti-rabbit-Cy3. A. LINGO-1 staining (red) is detected in sections of rat substantia nigra, in nigral neurons (arrows). B. Staining of rat spinal cord shows LINGO-1 labeling in ventral horn neurons (arrows). Cell nuclei are stained with DAPI (blue).
