Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- FAB1172N - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal ALCAM/CD166 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse ALCAM in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) ALCAM is observed, and less than 10% cross-reactivity with rhBCAM, recombinant mouse (rm) OCAM, and rmMAdCAM-1 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Near infrared dye
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of ALCAM/CD166 in Mouse Splenocytes by Flow Cytometry. Mouse Splenocytes either (A) activated or (B) resting were stained with Goat Anti-Mouse ALCAM/CD166 Alexa Fluor® 700-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1172N) and Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (Catalog # FAB1217P). Quadrant markers were set based on control antibody staining (Catalog # IC108N). View our protocol for Staining Membrane-associated Proteins.