Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- ELISA [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-37345 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal ALCAM/CD166 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Western blot analysis using ALCAM mouse mAb against NIH/3T3 cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Western blot analysis using ALCAM mAb against human ALCAM recombinant protein. (Expected MW is 44.9 kDa)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Western blot analysis using ALCAM mAb against HEK293 (1) and ALCAM (AA: 48-216)-hIgGFc transfected HEK293 (2) cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Tested antibody against two HT1080 samples: One sample is an RFP control and the other is an ALCAM knockout. Whole cell lysate separated on 1D 12% PAGE gel under 3 conditions: 1. Denatured, deglycosylated, reduced; 2. Denatured; 3. Denatured, reduced. Loading order: 1. Protein Ladder 2. HT1080 ALCAM-RFP (Denatured, deglycosylated, reduced) 3. HT1080 ALCAM-KO (Denatured, deglycosylated, reduced) 4. Protein Ladder 5. HT1080 ALCAM-RFP (Denatured) 6. HT1080 ALCAM-KO (Denatured) 7. Protein Ladder 8. HT1080 ALCAM-RFP (Denatured, reduced) 9. HT1080 ALCAM-KO (Denatured, reduced). This image was submitted via customer Review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Analysis of prostate cancer tissues using ALCAM mouse mAb with DAB staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Analysis of bladder cancer tissues using ALCAM mouse mAb with DAB staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Flow cytometric analysis of Jurkat cells using ALCAM mouse mAb (green) and negative control (red).