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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [1]
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- Product number
- FAB1172P - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse ALCAM/CD166 PE-conjugated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse ALCAM in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) ALCAM is observed, and less than 10% cross-reactivity with rhBCAM, recombinant mouse (rm) OCAM, and rmMAdCAM-1 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Antigen sequence
AAC06342- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Intestinal Enteroendocrine Lineage Cells Possess Homeostatic and Injury-Inducible Stem Cell Activity.
IRF4 controls the positioning of mature B cells in the lymphoid microenvironments by regulating NOTCH2 expression and activity.
Yan KS, Gevaert O, Zheng GXY, Anchang B, Probert CS, Larkin KA, Davies PS, Cheng ZF, Kaddis JS, Han A, Roelf K, Calderon RI, Cynn E, Hu X, Mandleywala K, Wilhelmy J, Grimes SM, Corney DC, Boutet SC, Terry JM, Belgrader P, Ziraldo SB, Mikkelsen TS, Wang F, von Furstenberg RJ, Smith NR, Chandrakesan P, May R, Chrissy MAS, Jain R, Cartwright CA, Niland JC, Hong YK, Carrington J, Breault DT, Epstein J, Houchen CW, Lynch JP, Martin MG, Plevritis SK, Curtis C, Ji HP, Li L, Henning SJ, Wong MH, Kuo CJ
Cell stem cell 2017 Jul 6;21(1):78-90.e6
Cell stem cell 2017 Jul 6;21(1):78-90.e6
IRF4 controls the positioning of mature B cells in the lymphoid microenvironments by regulating NOTCH2 expression and activity.
Simonetti G, Carette A, Silva K, Wang H, De Silva NS, Heise N, Siebel CW, Shlomchik MJ, Klein U
The Journal of experimental medicine 2013 Dec 16;210(13):2887-902
The Journal of experimental medicine 2013 Dec 16;210(13):2887-902
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of ALCAM/CD166 in Mouse Splenocytes by Flow Cytometry. Mouse Splenocytes either (A) activated or (B) resting were stained with Goat Anti-Mouse ALCAM/CD166 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1172P) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A). Quadrant markers were set based on control antibody staining (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.
