MA5-23565

antibody from Invitrogen Antibodies

Targeting: ALCAM CD166, MEMD

Provider product page for MA5-23565

Flow cytometry

Other assay

Antibody data

Antibody Data
Antigen structure
References [1]
Comments [0]
Validations
Flow cytometry [1]
Other assay [3]

Submit

Product number: MA5-23565 - Provider product page
Provider: Invitrogen Antibodies
Product name: CD166 Monoclonal Antibody (105902), Alexa Fluor™ 488
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: This antibody shows approximately 50% cross-reactivity with recombinant mouse OCAM and no cross-reactivity with recombinant human (rh) BCAM, rhEpCAM, rhMCAM, or rhNCAM-L1.
Reactivity: Human
Host: Mouse
Conjugate: Green dye
Isotype: IgG
Antibody clone number: 105902
Vial size: 100 Tests
Storage: 4° C, store in dark, DO NOT FREEZE!

ALCAM protein structure - MA5-23565 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 DGUOK is required for cancer cell stemness in lung adenocarcinoma cells A, B Flow cytometric assay using ALDEFLUOR (A) and anti-CD166 antibody (B) showing the effects of DGUOK depletion on the proportion of ALDH + cells (A) and CD166 + cells (B) in lung adenocarcinoma cell lines. C, D Representative images and quantitation of the effects of DGUOK depletion on lung tumor sphere formation by lung adenocarcinoma cells. E Cell survival assay using annexin V/PI staining and flow cytometric to determine the effects of DGUOK depletion on H1650 cell survival under adherent and non-adherent (30 h in lung sphere formation conditions) conditions. F Quantitation of apoptotic cells in (E) from three independent biological replicates. G, H Representative immunofluorescence staining showing the effects of DGUOK KO on expression of CK20 in Matrigel-embedded H1650 (G) and A549 (H) tumor spheres. FBS was added at day 5 to induce CD20 expression. Data information: Data are shown as mean +- SD. n = 3 in (D and F). P values were determined by two-tailed, two-sample Student's t -test. Experiments were repeated with three biological replicates with similar results.
Conjugate: Green dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure EV2 The effect of DGUOK KO on CSC population A Negative control for the ALDEFLUOR assay data in Fig 2 A. DEAB (diethylaminobenzaldehyde), an aldehyde dehydrogenase inhibitor, was used to inhibit ALDH in lung adenocarcinoma cells. B ALDEFLUOR and anti-CD166 antibody staining were employed to determine the effect of DGUOK KO on CD166 + /ALDH + population in H1650 cells. C Flow cytometric assay data indicating that all the H1650 cells were positive for CD49f expression. D Quantitation of the expression levels in control and DGUOK KO2 H1650 cells in (C). E The effect of DGUOK KO on the ""side population (SP)"" cells in H1650 and A549 cells, as determined by Hoechst blue and Hoechst red double staining. The SP cells disappeared when cells were treated with fumitremorgin C (FTC), an inhibitor for ABCG2. F The expression levels of DGUOK in H1650, A549, and PDCs. G The effects of DGUOK KO on tumor sphere formation in PDCs. Data are shown as mean +- SD from three technical replicates ( n = 3). P values were determined by two-tailed, two-sample Student's t -test.
Conjugate: Green dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6 DGUOK depletion regulates CSC self-renewal by inhibiting mitochondrial OXPHOS A, B The ectopic expression of yeast NDI1 (A) and DGUOK (B) in DGUOK KO H1650 and LLC cells was able to partially restore the levels of ALDH + CSC population, as determined by flow cytometric and ALDEFLUOR assay. C The ectopic expression NDI1 in GUOK KO H1650 cell was able to restore the CD166 + CSC population, as determined by flow cytometric. D, E The ectopic expression NDI1 (D) and DGUOK (E) was able to restore lung tumor sphere formation in DGUOK KO lung adenocarcinoma cells. Data are shown as mean +- SD ( n = 3 independent replicates per group). F, G Limiting dilution tumor initiation assay to determine the effects of DGUOK depletion and NDI1 rescue (F) or DGUOK rescue (G) on CSC self-renewal in LLC cells. H, I The effects of NDI1 (H) and ectopic DGUOK (I) in DGUOK KO LLC cells on tumor growth (1 x 10 5 cells). Data are shown as mean +- SD ( n = 8). Experiment in NDI alone group was ended on day 22 due to tumor ulceration. Other groups were ended on day 26. Left panel, tumor growth curves from each group of mice. Right panel, the image of harvested LLC allografts tumors after euthanasia of mice on day 26 (1 x 10 5 cell groups). Data information: P values in (D and E) were determined by two-tailed, two-sample t -test. Experiments in (A and B) were repeated with three biological replicates with similar results.
Conjugate: Green dye