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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MA5-23849 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD166 Monoclonal Antibody (105901)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In Western blots, no cross-reactivity with recombinant human (rh) BCAM, recombinant mouse OCAM, rhMCAM, or rhEpCAM is observed. Reconstitute at 0.5 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 105901
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of CD166 in HuT 78 human cutaneous T cell lymphoma cell line. Samples were incubated in CD166 monoclonal antibody (Product # MA5-23849) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for ALCAM/CD166 at approximately 120 kDa (as indicated). For best results, non-reducing conditions only are recommended.
