Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- A01788-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-CD166/ALCAM Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for CD166/ALCAM detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. CD166/ALCAM information: Molecular Weight: 65102 MW; Subcellular Localization: Membrane; Single-pass type I membrane protein; Tissue Specificity: Spleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Comparative Proteomic Analysis of tPVAT during Ang II Infusion.
Effect of fluid shear stress on cardiomyogenic differentiation of rat bone marrow mesenchymal stem cells.
Liang X, Guan H, Sun J, Qi Y, Yao W
Biomedicines 2021 Dec 2;9(12)
Biomedicines 2021 Dec 2;9(12)
Effect of fluid shear stress on cardiomyogenic differentiation of rat bone marrow mesenchymal stem cells.
Huang Y, Jia X, Bai K, Gong X, Fan Y
Archives of medical research 2010 Oct;41(7):497-505
Archives of medical research 2010 Oct;41(7):497-505
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of CD166/ALCAM using anti- CD166/ALCAM antibody (A01788-1). CD166/ALCAM was detected in paraffin-embedded section of mouse gaster tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- CD166/ALCAM Antibody (A01788-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image