46-1668-41

antibody from Invitrogen Antibodies
Targeting: ALCAM CD166, MEMD
Provider product page for 46-1668-41

Antibody data

Product number
46-1668-41 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-CD166 (ALCAM) Monoclonal Antibody (3A6), PerCP-eFluor 710, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: This 3A6 monoclonal antibody reacts with human CD166, which is also known as activated leukocyte cell adhesion molecule (ALCAM). CD166 is an immunoglobulin superfamily cell adhesion molecule that is broadly expressed on developing and adult hematopoietic (e.g., activated T and B cells, monocytes, and dendritic cells) and non-hematopoietic (e.g., neural, endothelial, and epithelial) tissues. CD166 has also been detected in melanoma, colorectal carcinoma, as well as cancers of the breast, prostate, and bladder. CD166 mediates cell-cell adhesion by forming low-affinity homophilic interactions (ALCAM-ALCAM) or high-affinity heterophilic interactions with its ligand CD6 (ALCAM-CD6). CD166 plays a role in development, inflammation, and neutrophil migration. Moreover, its expression can be used as a prognostic marker for several cancers and has been reported to be a cancer stem cell marker. The 3A6 antibody has been reported to induce the internalization of CD166 and its recycling back to the cell surface. Applications Reported: This 3A6 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 3A6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood lymphocytes. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
3A6
Vial size
25 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
ALCAM protein structure - 46-1668-41 shown in red.