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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Flow cytometry [1]
- Other assay [2]
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- Product number
- 46-1668-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD166 (ALCAM) Monoclonal Antibody (3A6), PerCP-eFluor™ 710, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 3A6 monoclonal antibody reacts with human CD166, which is also known as activated leukocyte cell adhesion molecule (ALCAM). CD166 is an immunoglobulin superfamily cell adhesion molecule that is broadly expressed on developing and adult hematopoietic (e.g., activated T and B cells, monocytes, and dendritic cells) and non-hematopoietic (e.g., neural, endothelial, and epithelial) tissues. CD166 has also been detected in melanoma, colorectal carcinoma, as well as cancers of the breast, prostate, and bladder. CD166 mediates cell-cell adhesion by forming low-affinity homophilic interactions (ALCAM-ALCAM) or high-affinity heterophilic interactions with its ligand CD6 (ALCAM-CD6). CD166 plays a role in development, inflammation, and neutrophil migration. Moreover, its expression can be used as a prognostic marker for several cancers and has been reported to be a cancer stem cell marker.
- Antibody clone number
- 3A6
- Concentration
- 5 µL/Test
Submitted references Cell surface galectin-3 defines a subset of chemoresistant gastrointestinal tumor-initiating cancer cells with heightened stem cell characteristics.
Enrichment of Human Stem-Like Prostate Cells with s-SHIP Promoter Activity Uncovers a Role in Stemness for the Long Noncoding RNA H19.
Comparison of human adipose-derived stem cells isolated from subcutaneous, omental, and intrathoracic adipose tissue depots for regenerative applications.
Mechanisms of transcriptional regulation and prognostic significance of activated leukocyte cell adhesion molecule in cancer.
Internalization and recycling of ALCAM/CD166 detected by a fully human single-chain recombinant antibody.
Expression and characterization of a novel CD6 ligand in cells derived from joint and epithelial tissues.
Molecular basis for the homophilic activated leukocyte cell adhesion molecule (ALCAM)-ALCAM interaction.
Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand.
Ilmer M, Mazurek N, Byrd JC, Ramirez K, Hafley M, Alt E, Vykoukal J, Bresalier RS
Cell death & disease 2016 Aug 11;7(8):e2337
Cell death & disease 2016 Aug 11;7(8):e2337
Enrichment of Human Stem-Like Prostate Cells with s-SHIP Promoter Activity Uncovers a Role in Stemness for the Long Noncoding RNA H19.
Bauderlique-Le Roy H, Vennin C, Brocqueville G, Spruyt N, Adriaenssens E, Bourette RP
Stem cells and development 2015 May 15;24(10):1252-62
Stem cells and development 2015 May 15;24(10):1252-62
Comparison of human adipose-derived stem cells isolated from subcutaneous, omental, and intrathoracic adipose tissue depots for regenerative applications.
Russo V, Yu C, Belliveau P, Hamilton A, Flynn LE
Stem cells translational medicine 2014 Feb;3(2):206-17
Stem cells translational medicine 2014 Feb;3(2):206-17
Mechanisms of transcriptional regulation and prognostic significance of activated leukocyte cell adhesion molecule in cancer.
King JA, Tan F, Mbeunkui F, Chambers Z, Cantrell S, Chen H, Alvarez D, Shevde LA, Ofori-Acquah SF
Molecular cancer 2010 Oct 7;9:266
Molecular cancer 2010 Oct 7;9:266
Internalization and recycling of ALCAM/CD166 detected by a fully human single-chain recombinant antibody.
Piazza T, Cha E, Bongarzone I, Canevari S, Bolognesi A, Polito L, Bargellesi A, Sassi F, Ferrini S, Fabbi M
Journal of cell science 2005 Apr 1;118(Pt 7):1515-25
Journal of cell science 2005 Apr 1;118(Pt 7):1515-25
Expression and characterization of a novel CD6 ligand in cells derived from joint and epithelial tissues.
Saifullah MK, Fox DA, Sarkar S, Abidi SM, Endres J, Piktel J, Haqqi TM, Singer NG
Journal of immunology (Baltimore, Md. : 1950) 2004 Nov 15;173(10):6125-33
Journal of immunology (Baltimore, Md. : 1950) 2004 Nov 15;173(10):6125-33
Molecular basis for the homophilic activated leukocyte cell adhesion molecule (ALCAM)-ALCAM interaction.
van Kempen LC, Nelissen JM, Degen WG, Torensma R, Weidle UH, Bloemers HP, Figdor CG, Swart GW
The Journal of biological chemistry 2001 Jul 13;276(28):25783-90
The Journal of biological chemistry 2001 Jul 13;276(28):25783-90
Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand.
Bowen MA, Patel DD, Li X, Modrell B, Malacko AR, Wang WC, Marquardt H, Neubauer M, Pesando JM, Francke U
The Journal of experimental medicine 1995 Jun 1;181(6):2213-20
The Journal of experimental medicine 1995 Jun 1;181(6):2213-20
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (blue histogram) or Anti-Human CD166 (ALCAM) PerCP-eFluor® 710 (purple histogram). Cells in the monocyte gate were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Surface Gal3 defines a subtype of epithelial CSC. ( a ) Colorectal cancer cells LiM6 and DLD1 were investigated by flow cytometry for cell surface markers CD24/CD44 (left). CD24+/CD44+-cells (in black) were then investigated for CD166/EpCAM-expression (middle) and CD24+/CD44+/CD166+/EpCAM+-positive cells (in black middle) for Gal3-expression (right panel), where ++++ Gal3 - in green refers to cells positive for CD24, CD44, CD166, and EpCAM, but negative for Gal3, and ++++ Gal3 + in red refers to cells positive for CD24, CD44, CD166, EpCAM, and Gal3. ( b ) Pancreatic cancer cell lines AsPC1 and L3.6pl were subjected to the same analysis as in ( a )
