MA5-38443

antibody from Invitrogen Antibodies

Targeting: ALCAM CD166, MEMD

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-38443

Provider

Invitrogen Antibodies

Product name

CD166 Monoclonal Antibody (8E12C7)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

This antibody has been tested in indirect ELISA.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

8E12C7

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CD166 (ALCAM) using CD166 Monoclonal Antibody (Product # MA5-38443) in HEK293 (1) and ALCAM (AA: 48-216)-human IgG-Fc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis using CD166 Monoclonal Antibody (Product # MA5-38443) against human ALCAM recombinant protein. (Expected MW is 44.9 kDa)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CD166 (ALCAM) using CD166 Monoclonal Antibody (Product # MA5-38443) in HEK293 (1) and ALCAM (AA: 48-216)-human IgG-Fc transfected HEK293 (2) cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of CD166 (ALCAM) in HeLa cells using CD166 Monoclonal Antibody (Product # MA5-38443) (green). Blue: DRAQ5 fluorescent DNA dye.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of CD166 (ALCAM) in JURKAT cells using CD166 Monoclonal Antibody (Product # MA5-38443) (green) and negative control (red).