LS-C397916

antibody from LSBio

Targeting: GCDH ACAD5

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C397916

Provider

LSBio

Product name

GCD / GCDH Antibody LS-C397916

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot All lanes: GCDH antibody at 8µg/ml Lane 1: Mouse kidney tissue Lane 2: Mouse liver tissue Lane 3: Hela whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: LO2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 49, 48 kDa Observed band size: 49 kDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating GCDH in Hela whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of GCDH Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: GCDH Antibody (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating GCDH in Hela whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of GCDH Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: GCDH Antibody (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating GCDH in Hela whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of GCDH Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: GCDH Antibody (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg)