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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [6]
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- Product number
- PA5-60294 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GCDH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunogen sequence: SRPEFDWQDP LVLEEQLTTD EILIRDTFRT YCQERLMPRI LLANRNEVFH REIISEMGEL GVLGPTIKGY GCAGV Highest antigen sequence identity to the following orthologs: Mouse - 87%, Rat - 88%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Deglutarylation of glutaryl-CoA dehydrogenase by deacylating enzyme SIRT5 promotes lysine oxidation in mice.
Bhatt DP, Mills CA, Anderson KA, Henriques BJ, Lucas TG, Francisco S, Liu J, Ilkayeva OR, Adams AE, Kulkarni SR, Backos DS, Major MB, Grimsrud PA, Gomes CM, Hirschey MD
The Journal of biological chemistry 2022 Apr;298(4):101723
The Journal of biological chemistry 2022 Apr;298(4):101723
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of GCDH in human cell line U-2 OS shows positivity in mitochondria. Samples were probed using a GCDH Polyclonal Antibody (Product # PA5-60294).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorecent analysis of GCDH in human cell line U-2 OS using GCDH Polyclonal Antibody (Product # PA5-60294). Staining shows positivity in mitochondria.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GCDH in human liver using a GCDH Polyclonal Antibody (Product # PA5-60294) shows strong cytoplasmic positivity in hepatocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GCDH in human kidney, liver, pancreas and testis using GCDH Polyclonal Antibody (Product # PA5-60294) (A) shows similar protein distribution across tissues to an independent GCDH Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GCDH in human pancreas using GCDH Polyclonal Antibody (Product # PA5-60294) shows moderate granular cytoplasmic positivity in exocrine glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GCDH in human kidney using GCDH Polyclonal Antibody (Product # PA5-60294) shows strong granular cytoplasmic positivity in cells in tubules.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GCDH in human testis using GCDH Polyclonal Antibody (Product # PA5-60294) shows weak granular cytoplasmic positivity.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GCDH in human liver using GCDH Polyclonal Antibody (Product # PA5-60294) shows strong granular cytoplasmic positivity in hepatocytes.
