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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA5-30040 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GCDH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2. Predicted reactivity: Mouse (88%), Rat (89%), Xenopus laevis (84%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GCDH Polyclonal Antibody (Product # PA5-30040). Sample (30 µg of whole cell lysate). Lane A: Hep G2. 10% SDS PAGE. GCD antibody . GCDH Polyclonal Antibody (Product # PA5-30040) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GCDH Polyclonal Antibody detects GCDH protein at Mitochondria by immunofluorescent analysis. Sample: HepG2 cells were fixed in -20ºC 100% MeOH for 5 min. Green: GCDH protein stained by GCDH Polyclonal Antibody (Product # PA5-30040) diluted at 1:500. Blue: Hoechst 33343 staining.
