A301-274A

antibody from Invitrogen Antibodies

Targeting: INTS11 CPSF3L, CPSF73L, FLJ20542, INT11, RC-68

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-274A

Provider

Invitrogen Antibodies

Product name

INT11 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse INT11 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, K-562, A-549, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INT11 antibody (Product # A301-274A; lot A301-274A-4) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human INT11 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-INT11 antibody A301-274A (lot A301-274A-3) used for IP at 6 µg per reaction. INT11 was also immunoprecipitated by a previous lot of this antibody (lot A301-274A-2) and rabbit anti-INT11 antibody BL5185. For blotting immunoprecipitated INT11, A301-274A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse INT11 by western blot. Samples: Whole cell lysate from HeLa (15 µg), 293T (50 µg), mouse TCMK-1 (50 µg), and mouse NIH3T3 (50 µg) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INT11 antibody A301-274A (lot A301-274A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human INT11 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-INT11 (Product # A301-274A; lot 4) used at a dilution of 1:5,000 (0.2 µg/mL). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human INT11 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-INT11 (Cat. No. A301-274A Lot3) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human INT11 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-INT11 antibody (Product # A301-274A; lot A301-274A-4) used for IP at 6 µg per reaction. INT11 was also immunoprecipitated by a previous lot of this antibody (lot A301-274A-3). For blotting immunoprecipitated INT11 (Product # A301-274A) was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.