NB100-60638
antibody from Novus Biologicals
Targeting: INTS11
CPSF3L, CPSF73L, FLJ20542, INT11, RC-68
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NB100-60638 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-60638, RRID:AB_2276617
- Product name
- Rabbit Polyclonal INTS11 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: INTS11 Antibody [NB100-60638] - analysis of INTS11 in HeLa whole cell extracts (samples treated with siCtrl or siINTS11) using anti-INTS11 antibody. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: INTS11 Antibody [NB100-60638] - Detection of Human and Mouse INT11 by Western Blot. Samples: Whole cell lysate from HeLa (15 ug), 293T (50 ug), mouse TCMK-1 (50 ug), and mouse NIH3T3 (50 ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INT11 antibody NB100-60638 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: INTS11 Antibody [NB100-60638] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. NB100-60638 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: INTS11 Antibody [NB100-60638] - Whole cell lysate (50 ug) from HeLa, K-562, A-549, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INT11 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: INTS11 Antibody [NB100-60638] - Detection of human INT11 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-INT11 antibody NB100-60638 (lot NB100-60638-3) used for IP at 6 ug per reaction. INT11 was also immunoprecipitated by a previous lot of this antibody (lot NB100-60638) and another rabbit anti-INT11 antibody. For blotting immunoprecipitated INT11, NB100-60638 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: INTS11 Antibody [NB100-60638] - Human breast carcinoma. Antibody: Affinity purified rabbit anti-INT11 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: INTS11 Antibody [NB100-60638] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-INT11 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB