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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- AM31793PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM31793PU-N, RRID:AB_10975948
- Product name
- anti HADH / HCDH
- Antibody type
- Monoclonal
- Antigen
- HADH antibody was raised against hADHSC (NP_005318, 205 a.a. ~ 315 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4B5
- Vial size
- 50 µg
- Concentration
- 0,5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot analysis of HADH expression in HepG2 cell lysate.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot analysis of HADH expression in transfected 293T cell line by HADH monoclonal antibody.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged HADHSC is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to HADHSC on HeLa cell. [antibody concentration 10 µg/ml]
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunoprecipitation of HADH transfected lysate using anti-HADH monoclonal antibody and Protein A Magnetic Bead.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)
