Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-27352 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GALC Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Galactosylceramidase-transfected 293T. Predicted reactivity: Dog (87%), Pig (87%), Rhesus Monkey (95%), Bovine (84%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.46 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Galactosylceramidase using 30 µg of H1299 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Galactosylceramidase polyclonal antibody (Product # PA5-27352) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GALC was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a GALC Polyclonal Antibody (Product # PA5-27352) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GALC Polyclonal Antibody (Product # PA5-27352). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GALC Polyclonal Antibody (Product # PA5-27352) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of GALC was performed in paraffin-embedded human ovarian cancer tissue using GALC Polyclonal Antibody (Product # PA5-27352) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.