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- Product number
- PA5-29236 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MEK5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: U87-MG. Predicted reactivity: Mouse (98%), Rat (98%), Chicken (91%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Astrocyte-specific overexpressed gene signatures in response to methamphetamine exposure in vitro.
Bortell N, Basova L, Semenova S, Fox HS, Ravasi T, Marcondes MC
Journal of neuroinflammation 2017 Mar 9;14(1):49
Journal of neuroinflammation 2017 Mar 9;14(1):49
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Fig. 2 Validation of selected upregulated genes. a Transcriptional levels of MAP2K5, GRP65, and CXCL5 in astrocytes stimulated with 1, 10, and 100 muM of Meth for 24 h, examined by SyBrGreen qRT-PCR. Results are the Mean +- SEM of three independent experiments performed in duplicate. b Representative western blots for detection of MEK5, the protein encoded by MAP2K5 gene, GPR65, and normalizing b-actin, in protein extracts from astrocytes stimulated with 1, 10, and 100 muM of Meth for 24 h. c Normalized band intensity was calculated in ImageJ software (NIH). d Confocal imaging showing representative astrocytic cultures stained with specific antibodies for detection of MEK5, GPR65, and CXCL5 in 10 and 100 muM Meth treatments. e Fluorescence intensity of the expression of MEK5, GPR65, and CXCL5 was calculated in ImageJ (NIH) for cultures stimulated with 1, 10, and 100 muM, as well as controls. * p < 0.05 in one-way ANOVA followed by Bonferroni's post hoc comparison against control conditions
