MA5-56066

antibody from Invitrogen Antibodies

Targeting: DGKZ DAGK5, DAGK6, DGK-ZETA, hDGKzeta

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-56066

Provider

Invitrogen Antibodies

Product name

DGKZ Monoclonal Antibody (K49027_6B9)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Sequence of this protein is as follows: STGSKDVVRY LLDHAPPEIL DAVEENGETC LHQAAALGQR TICHYIVEAG ASLMKTDQQG DTPRQRAEKA QDTELAAYLE NRQHYQMIQR

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

K49027_6B9

Vial size

200 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DGKZ in 15 µg of mouse brain tissue. Sample was run on 6-18% SDS-PAGE under reducing conditions, blotted onto nitrocellulose membrane, and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. DGKZ band was visualized using ECL Substrate. Incubation with primary DGKZ monoclonal antibody (Product # MA5-55686) at a dilution of 1 µg/mL was used.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of DGKZ in 200 µg of mouse brain tissue lysate. Samples are as follows: Lane 1: Mouse brain tissue lysate, Lane 2: DGKZ immunoprecipitated from mouse brain tissue lysate, Lane3: The same as Lane 2 but KT82 was used as IgG isotype control antibody. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE, blotted onto nitrocellulose membrane, and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. The isotype control antibody was KT82. Incubation of samples with DGKZ monoclonal antibody (Product # MA5-55686) at a dilution of 2.5 µg was used.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of DGKZ in paraffin-embedded cerebellum tissue. Sample was incubated with DGKZ monoclonal antibody (Product # MA5-55686) at a dilution of 5 µg/mL (RT, 1 hour). Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.