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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-54437 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZBTB5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GEKEHMRVVV KSEPLSSPEP QDEVSDVTSQ AEGSESVEVE GVVVSAEKID LSPESSDRSF SDPQSSTDRV GDIHILEVTN NLEHKSTFSI SNFLNKSRGN NFTANQNN Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 93%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ZBTB5 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a ZBTB5 Polyclonal Antibody (Product # PA5-54437).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ZBTB5 in human cell line SH-SY5Y shows positivity in the Golgi apparatus & nucleus but excluded from the nucleoli. Samples were probed using a ZBTB5 Polyclonal Antibody (Product # PA5-54437).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ZBTB5 in human cell line HeLa using a ZBTB5 Polyclonal Antibody (Product # PA5-54437) shows localization to nucleoplasm and nuclear bodies.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ZBTB5 in human testis tissue shows strong cytoplasmic and nuclear positivity in seminiferus ducts cells. Samples were probed using a ZBTB5 Polyclonal Antibody (Product # PA5-54437).
