Explore
Validate
Learn
Immunohistochemistry
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-34060 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- OSTC Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Mouse, Rat, Hamster, Panda, Bovine, Dog, Pig, Opossum, Xenopus (94%).
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references DC2 and KCP2 mediate the interaction between the oligosaccharyltransferase and the ER translocon.
Shrimal S, Cherepanova NA, Gilmore R
The Journal of cell biology 2017 Nov 6;216(11):3625-3638
The Journal of cell biology 2017 Nov 6;216(11):3625-3638
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3. OST assays of microsomes isolated from siRNA-treated STT3B -/- cells. STT3B -/- cells were treated with a negative control siRNA (NC) or siRNAs specific for STT3A, DC2, or KCP2. (A) Cell extracts were resolved by SDS-PAGE for protein immunoblot analysis. (B) After 48 h of siRNA treatment, cells were retransfected with the siRNA plus pSAP-DDKHis or Tf-DDKHis expression vectors. After 24 h, pulse-labeled proteins were immunoprecipitated using anti-DDK sera. EH, endoglycosidase H. Numbered arrows designate the number of N-linked glycans. (C) A buffer control (-) or solubilized HEK293-derived microsomes were assayed for OST activity using bovine Dol-PP-OS as the donor substrate and a fluorescent peptide as the acceptor substrate. The glycopeptide product (peptide-GlcNAc 2 Man 9 Glc 3 , G3-peptide) was separated from the peptide by gel electrophoresis. Protein immunoblot analysis using anti-Sec61alpha of microsomes that were used for the OST assays. Quantified values below gel lanes are the mean of either two (A and B) or three (C) determinations.
