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Immunohistochemistry
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- PA5-33022 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLC11A2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Percent identity with other species by BLAST analysis: Human, Gibbon (100%) Gorilla, Monkey, Bovine, Elephant, Pig (93%) Dog, Rabbit (87%) Marmoset, Panda (80%).
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Iron Prevents Hypoxia-Associated Inflammation Through the Regulation of Nuclear Factor-κB in the Intestinal Epithelium.
Simmen S, Cosin-Roger J, Melhem H, Maliachovas N, Maane M, Baebler K, Weder B, Maeyashiki C, Spanaus K, Scharl M, de Vallière C, Zeitz J, Vavricka SR, Hausmann M, Rogler G, Ruiz PA
Cellular and molecular gastroenterology and hepatology 2019;7(2):339-355
Cellular and molecular gastroenterology and hepatology 2019;7(2):339-355
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Figure 1 Hypoxia promotes iron mobilization from the intestinal mucosa. ( A-C ) Human subjects (n = 10) were exposed to hypoxic conditions in a hypobaric chamber simulating an altitude of 4000 meters above sea level for 3 hours. Distal colon biopsy specimens were taken the day before entering the hypobaric chamber (T0), immediately after hypoxia (T1), and 1 week after collection of the first biopsy (T2). Immunostaining for ( A ) ferritin, ( B ) DMT-1, and ( C ) FPN was performed and the areas of positive staining were quantified and normalized to nuclei staining. Results represent means + SEM. * P < .05; ** P < .01. Scale bars : 25 mum. ( D ) Iron content in colon biopsy specimens also was assessed by atomic absorption spectrometry. ( E-H ) Transcript analysis for ( E ) ferritin, ( F ) DMT-1, ( G ) ferroportin, and ( H ) IL6 was performed. ( I-L ) Serum samples were collected at the time of screening (S), the day before entering the hypobaric chamber (baseline, T0), and 1 day (T1), 1 week (T2), and 1 month (T3) after baseline. Laboratory analysis of ( I ) serum iron, ( J ) Tf saturation, ( K ) Tf, and ( L ) ferritin was performed. Results represent means + SEM.
