AP1242a

antibody from Abgent

Targeting: PIAS1 DDXBP1, GBP, GU/RH-II, ZMIZ3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP1242a

Provider

Abgent

Proper citation

Abgent Cat#AP1242a, RRID:AB_2164062

Product name

PIAS1 Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"HEK293 cell extracts were prepared and immunoprecipitated with AP1242a (left lane alpha-PIAS1 IP) or a control antibody (right lane Control IP), followed by Western blotting with AP1242a. Taken from Polyakova o, Borman S, Grimley R, Vamathevan J, Hayes B. et al. (2012) Identification of Novel interacting Partners of Sirtuin6. Plos ONE 7 (12): e51555. doi: 10.1371/journal.pone. 0051555"

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of PIAS1 (arrow) using rabbit polyclonal PIAS1 Antibody (N-term) (RB00668). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIAS1 gene (Lane 2) (Origene Technologies).

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."

Primary Ab dilution

1:50~100