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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB1850 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1850, RRID:AB_1578728
- Product name
- PIAS1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PIAS1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references PIAS1 and PIASxalpha function as SUMO-E3 ligases toward androgen receptor and repress androgen receptor-dependent transcription.
Protein inhibitors of activated STAT resemble scaffold attachment factors and function as interacting nuclear receptor coregulators.
Sumoylation of Mdm2 by protein inhibitor of activated STAT (PIAS) and RanBP2 enzymes.
Nishida T, Yasuda H
The Journal of biological chemistry 2002 Nov 1;277(44):41311-7
The Journal of biological chemistry 2002 Nov 1;277(44):41311-7
Protein inhibitors of activated STAT resemble scaffold attachment factors and function as interacting nuclear receptor coregulators.
Tan JA, Hall SH, Hamil KG, Grossman G, Petrusz P, French FS
The Journal of biological chemistry 2002 May 10;277(19):16993-7001
The Journal of biological chemistry 2002 May 10;277(19):16993-7001
Sumoylation of Mdm2 by protein inhibitor of activated STAT (PIAS) and RanBP2 enzymes.
Miyauchi Y, Yogosawa S, Honda R, Nishida T, Yasuda H
The Journal of biological chemistry 2002 Dec 20;277(51):50131-6
The Journal of biological chemistry 2002 Dec 20;277(51):50131-6
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The PIAS1 polyclonal antibody (Cat # PAB1850) is used in Western blot to detect PIAS1 in HL-60 tissue lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PIAS1 (arrow) using PIAS1 polyclonal antibody (Cat # PAB1850). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIAS1 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PIAS1 polyclonal antibody (Cat # PAB1850), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
