ABIN500489

antibody from antibodies-online

Targeting: PIAS1 DDXBP1, GBP, GU/RH-II, ZMIZ3

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN500489

Provider

antibodies-online

Product name

anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (C-Term) antibody

Antibody type

Polyclonal

Antigen

15 amino acid peptide near the carboxy terminus of Human PIAS1 (AP30666CP-N).

Description

Affinity chromatography purified via peptide column

Reactivity

Human, Mouse, Rat

Host

Rabbit

Epitope

C-Term

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/mL

Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.

Handling

Avoid repeated freezing and thawing.

References

Submitted references

PIAS1 regulates CP2c localization and active promoter complex formation in erythroid cell-specific alpha-globin expression.

Kang HC, Chae JH, Jeon J, Kim W, Ha DH, Shin JH, Kim CG, Kim CG
Nucleic acids research 2010 Sep;38(16):5456-71

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Regulation of gene-activation pathways by PIAS proteins in the immune system.

Shuai K, Liu B
Nature reviews. Immunology 2005 Aug;5(8):593-605

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Inhibition of Stat1-mediated gene activation by PIAS1.

Liu B, Liao J, Rao X, Kushner SA, Chung CD, Chang DD, Shuai K
Proceedings of the National Academy of Sciences of the United States of America 1998 Sep 1;95(18):10626-31

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western blot analysis of PIAS1 in human kidney tissue lysate with PIAS1 antibody cat.-No AP30666PU-N at 1 ?g/ml in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Immunohistochemistry of PIAS1 in rat kidney tissue with PIAS1 antibody Cat.-No AP30666PU-N at 5 ?g/ml