23395-1-AP

antibody from Invitrogen Antibodies

Targeting: PIAS1 DDXBP1, GBP, GU/RH-II, ZMIZ3

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

23395-1-AP

Provider

Invitrogen Antibodies

Product name

PIAS1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: PSAKRTCPS LSPTSPLNNK GILSLPHQAS PVSRTPSLPA VDTSYINTSL IQDYRHPFHM TPMPYDLQGL DFFPFLSGDN QHYNTSLLAA AAAAVSDDQD LLHSSRFFPY TSSQMFLDQL SAGGSTSLPT TNGSSSGSNS SLVSSNSLRE SHSHTVTNRS STDTASIFGI IPDIISLD (475-651 aa encoded by BC121797)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.49 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 23395-1-AP (PIAS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 23395-1-AP (PIAS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 23395-1-AP (PIAS1 antibody) at dilution of 1:400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 23395-1-AP (PIAS1 antibody) at dilution of 1:400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PIAS1 (IP:23395-1-AP, 4ug; Detection:23395-1-AP 1:500) with HepG2 cells lysate 2520 ug.