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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AP11245PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP11245PU-N, RRID:AB_1770538
- Product name
- anti PIAS1 (N-term)
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PIAS1.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- (LEFT) Western blot analysis of PIAS1 polyclonal antibody (Cat.#AP11245PU-N) to detect PIAS1 in HL-60 tissue lysate. (RIGHT) Western blot analysis of PIAS1 (arrow) using rabbit polyclonal PIAS1 Antibody (N-term) (Cat.#AP11245PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIAS1 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
