Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [5]
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Validation data
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- Product number
- Ab099911 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Dishevelled/Dvl1 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit anti Human Dishevelled/Dvl1 Antibody, Polyclonal, could be used for WB, IHC and so on.Application:WB: 0.01-2µg/mLIHC: 5-20µg/mLProtein FunctionParticipates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. Required for LEF1 activation upon WNT1 and WNT3A signaling. DVL1 and PAK1 form a ternary complex with MUSK which is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ).
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 150-285
- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Dishevelled/Dvl1 Antibody (Ab099911) - Western Blot All lanes: Dishevelled/Dvl1 Antibody (Ab099911) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution Predicted band size: 75 kDa Observed band size: 75 kDa Exposure time: 100.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Dishevelled/Dvl1 Antibody (Ab099911) - IHC DAB staining on IHC Samples: Human Glioma Tissue Primary Ab: 20 µg/mL Dishevelled/Dvl1 Antibody (Ab099911) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Dishevelled/Dvl1 Antibody (Ab099911) - IHC DAB staining on IHC Sample: Mouse Colon Tissue Primary Ab: 10 µg/mL Dishevelled/Dvl1 Antibody (Ab099911) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Dishevelled/Dvl1 Antibody (Ab099911) - IHC DAB staining on IHC Sample: Mouse Cerebrum Tissue Primary Ab: 10 µg/mL Dishevelled/Dvl1 Antibody (Ab099911) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Dishevelled/Dvl1 Antibody (Ab099911) - IHC DAB staining on IHC Sample: Mouse Liver Tissue Primary Ab: 10 µg/mL Dishevelled/Dvl1 Antibody (Ab099911) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Dishevelled/Dvl1 Antibody (Ab099911) - IHC DAB staining on IHC Sample: Mouse Kidney Tissue Primary Ab: 10 µg/mL Dishevelled/Dvl1 Antibody (Ab099911) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.