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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00003133-D01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003133-D01, RRID:AB_10718754
- Product name
- HLA-E MaxPab rabbit polyclonal antibody (D01)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human HLA-E protein.
- Antigen sequence
MVDGTLLLLLSEALALTQTWAGSHSLKYFHTSVSR
PGRGEPRFISVGYVDDTQFVRFDNDAASPRMVPRA
PWMEQEGSEYWDRETRSARDTAQIFRVNLRTLRGY
YNQSEAGSHTLQWMHGCELGPDRRFLRGYEQFAYD
GKDYLTLNEDLRSWTAVDTAAQISEQKSNDASEAE
HQRAYLEDTCVEWLHKYLEKGKETLLHLEPPKTHV
THHPISDHEATLRCWALGFYPAEITLTWQQDGEGH
TQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTC
HVQHEGLPEPVTLRWKPASQPTIPIVGIIAGLVLL
GSVVSGAVVAAVIWRKKSSGGKGGSYSKAEWSDSA
QGSESHSL- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of HLA-E expression in transfected 293T cell line (H00003133-T02) by HLA-E MaxPab polyclonal antibody.Lane 1: HLA-E transfected lysate(40.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of HLA-E transfected lysate using anti-HLA-E MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HLA-E MaxPab mouse polyclonal antibody (B01) (H00003133-B01).
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
