Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 14-9953-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HLA-E Monoclonal Antibody (3D12HLA-E), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody 3D12 (HLA-E) recognizes human HLA-E, a member of the Human Leukocyte Antigen family. HLA-E is a MHC Class IB member, which has a specialized role in NK cell recognition. Binding of a restricted set of peptides derived from other Class I leader sequences to the HLA-E and B2M (beta2 microglobulin) complex leads to interaction with NKG2A on NK cells. This binding protects the HLA-G-expressing cell from NK cell killing. HLA-E expression is ubiquitous. Applications Tested: This 3D12 (HLA-E) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3D12HLA-E
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references High-efficiency lysis of cervical cancer by allogeneic NK cells derived from umbilical cord progenitors is independent of HLA status.
HLA-E-expressing pluripotent stem cells escape allogeneic responses and lysis by NK cells.
EBV BILF1 evolved to downregulate cell surface display of a wide range of HLA class I molecules through their cytoplasmic tail.
Restoration of TLR3-activated myeloid dendritic cell activity leads to improved natural killer cell function in chronic hepatitis B virus infection.
Selenite induces posttranscriptional blockade of HLA-E expression and sensitizes tumor cells to CD94/NKG2A-positive NK cells.
Cutting edge: HLA-E binds a peptide derived from the ATP-binding cassette transporter multidrug resistance-associated protein 7 and inhibits NK cell-mediated lysis.
HLA-E allelic variants. Correlating differential expression, peptide affinities, crystal structures, and thermal stabilities.
HLA-E is a major ligand for the natural killer inhibitory receptor CD94/NKG2A.
Veluchamy JP, Heeren AM, Spanholtz J, van Eendenburg JD, Heideman DA, Kenter GG, Verheul HM, van der Vliet HJ, Jordanova ES, de Gruijl TD
Cancer immunology, immunotherapy : CII 2017 Jan;66(1):51-61
Cancer immunology, immunotherapy : CII 2017 Jan;66(1):51-61
HLA-E-expressing pluripotent stem cells escape allogeneic responses and lysis by NK cells.
Gornalusse GG, Hirata RK, Funk SE, Riolobos L, Lopes VS, Manske G, Prunkard D, Colunga AG, Hanafi LA, Clegg DO, Turtle C, Russell DW
Nature biotechnology 2017 Aug;35(8):765-772
Nature biotechnology 2017 Aug;35(8):765-772
EBV BILF1 evolved to downregulate cell surface display of a wide range of HLA class I molecules through their cytoplasmic tail.
Griffin BD, Gram AM, Mulder A, Van Leeuwen D, Claas FH, Wang F, Ressing ME, Wiertz E
Journal of immunology (Baltimore, Md. : 1950) 2013 Feb 15;190(4):1672-84
Journal of immunology (Baltimore, Md. : 1950) 2013 Feb 15;190(4):1672-84
Restoration of TLR3-activated myeloid dendritic cell activity leads to improved natural killer cell function in chronic hepatitis B virus infection.
Tjwa ET, van Oord GW, Biesta PJ, Boonstra A, Janssen HL, Woltman AM
Journal of virology 2012 Apr;86(8):4102-9
Journal of virology 2012 Apr;86(8):4102-9
Selenite induces posttranscriptional blockade of HLA-E expression and sensitizes tumor cells to CD94/NKG2A-positive NK cells.
Enqvist M, Nilsonne G, Hammarfjord O, Wallin RP, Björkström NK, Björnstedt M, Hjerpe A, Ljunggren HG, Dobra K, Malmberg KJ, Carlsten M
Journal of immunology (Baltimore, Md. : 1950) 2011 Oct 1;187(7):3546-54
Journal of immunology (Baltimore, Md. : 1950) 2011 Oct 1;187(7):3546-54
Cutting edge: HLA-E binds a peptide derived from the ATP-binding cassette transporter multidrug resistance-associated protein 7 and inhibits NK cell-mediated lysis.
Wooden SL, Kalb SR, Cotter RJ, Soloski MJ
Journal of immunology (Baltimore, Md. : 1950) 2005 Aug 1;175(3):1383-7
Journal of immunology (Baltimore, Md. : 1950) 2005 Aug 1;175(3):1383-7
HLA-E allelic variants. Correlating differential expression, peptide affinities, crystal structures, and thermal stabilities.
Strong RK, Holmes MA, Li P, Braun L, Lee N, Geraghty DE
The Journal of biological chemistry 2003 Feb 14;278(7):5082-90
The Journal of biological chemistry 2003 Feb 14;278(7):5082-90
HLA-E is a major ligand for the natural killer inhibitory receptor CD94/NKG2A.
Lee N, Llano M, Carretero M, Ishitani A, Navarro F, López-Botet M, Geraghty DE
Proceedings of the National Academy of Sciences of the United States of America 1998 Apr 28;95(9):5199-204
Proceedings of the National Academy of Sciences of the United States of America 1998 Apr 28;95(9):5199-204
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with 0.5 µg of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.5 µg of Anti-Human HLA-E Purified (filled histogram) followed by Anti-Mouse IgG Biotin (Product # 13-4013-85)and Streptavidin PE (Product # 12-4317-87).Cells in the lymphocyte gate were used for analysis.