Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LF-MA0208 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-BID Monoclonal Antibody (21F10)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- A suggested positive control for this product is A431 cells.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 21F10
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot was performed using Anti-BID Monoclonal Antibody (21F10) (Product # LF-MA0208) and a 20 kDa band corresponding to BID was observed across cell lines and tissue extracts tested and decreased upon Staurosporine and also with Actinomycin D along with TNF Alpha treatment. The expression of BID is reported to be low in Mouse Brain compared to Mouse Spleen and Mouse Thymus. Fig.a shows whole cell extracts (30 µg lysate) of A-431 (Lane 1), A-431 treated with Staurosporine (1uM for 4 hr) (Lane 2), A-431 treated with Staurosporine (1uM for 8 hr) (Lane 3) and A-431 treated with Actinomycin along with TNF Alpha (5ug/ml and 10ng/ml for 5 hr) (Lane 4), Fig.b sows whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Jurkat (Lane 2), MOLT-4 (Lane 3), HeLa (Lane 4), PC-3 (Lane 5), SH-SY5Y (Lane 6) and tissue extracts of Mouse Spleen (Lane 7), Mouse Thymus (Lane 8) and Mouse Brain (Lane 9) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 ug/ml) and detected by chemiluminescence Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection with Novex® ECL Chemiluminescent Substrate Reagent.