Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-17034 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BID Monoclonal Antibody (3C5)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17034 targets BID in FACS, ICC, IHC, IF and WB applications and shows reactivity with Human samples.
- Antibody clone number
- 3C5
- Concentration
- Conc. Not Determined
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BID using BID monoclonal antibody (Product # MA5-17034) in HeLa (1), A431 (2), Jurkat (3), A549 (4), HepG2 (5), and HEK293 (6) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of BID was achieved by transfecting Hep G2 cells with BID specific siRNAs (Silencer® select Product # s1986). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the BID knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with BID Monoclonal Antibody (Product # MA5-17034, 1:2000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to BID.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts of Hep G2 (Lane 1), Jurkat (Lane 2), MCF7 (Lane 3), PANC-1 (Lane 4), A-431 (Lane 5), HeLa (Lane 6), MOLT4 (Lane 7), SH-SY5Y (Lane 8) and PC-3 (Lane 9). The blot was probed with Anti-BID Monoclonal Antibody (3C5) (Product # MA5-17034, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/ml, 1:4000 dilution). A 20 kDa band corresponding to BID was observed across the cell lines tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using BID monoclonal antibody (Product # MA5-17034) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded prostate tissues (left) and tonsil tissues (right) using BID monoclonal antibody (Product # MA5-17034) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using BID monoclonal antibody (Product # MA5-17034) (green) and negative control (purple).
