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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB10503 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10503, RRID:AB_11189520
- Product name
- BID monoclonal antibody, clone 3C5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant BID.
- Isotype
- IgG
- Antibody clone number
- 3C5
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using BID monoclonal antibody, clone 3C5 (Cat # MAB10503) against HeLa (1), A-431 (2), Jurkat (3), A-549 (4), HepG2 (5), and HEK293 (6) cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA detection with BID monoclonal antibody, clone 3C5 (Cat # MAB10503).
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using BID monoclonal antibody, clone 3C5 (Cat # MAB10503) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human prostate tissues (A) and tonsil tissues (B) using BID monoclonal antibody, clone 3C5 (Cat # MAB10503) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using BID monoclonal antibody, clone 3C5 (Cat # MAB10503) (green) and negative control (purple).
- Validation comment
- Flow Cytometry
