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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [2]
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- Product number
- LS-C98846 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-C98846, RRID:AB_2228096
- Product name
- BID Antibody (aa68-103) LS-C98846
- Antibody type
- Polyclonal
- Description
- Ammonium sulfate precipitation
- Reactivity
- Human
- Host
- Rabbit
- Storage
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- The anti-Bid BH3 domain antibody is used in Western blot to detect Bid BH3 in HL-60 cell lysate.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of anti-hBid-BH3 antibody in mouse lung tissue lysates (35 ug/lane). hBid-BH3(arrow) was detected using the purified antibody.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of Bid (arrow) using rabbit polyclonal Bid Antibody (BH3). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Bid gene.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of Bid (arrow) using rabbit polyclonal Bid Antibody (BH3). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Bid gene.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Bid BH3 Domain Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
