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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
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- Product number
- AP1307a - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP1307a, RRID:AB_2065620
- Product name
- Bid Antibody (BH3 Domain Specific)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 1.855 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references ALS-linked mutant SOD1 damages mitochondria by promoting conformational changes in Bcl-2.
Pedrini S, Sau D, Guareschi S, Bogush M, Brown RH Jr, Naniche N, Kia A, Trotti D, Pasinelli P
Human molecular genetics 2010 Aug 1;19(15):2974-86
Human molecular genetics 2010 Aug 1;19(15):2974-86
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- The anti-Bid BH3 domain Pab (Cat. #AP1307a) is used in Western blot to detect Bid BH3 in HL-60 cell lysate.
- Primary Ab dilution
- 1:1000
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Western blot analysis of anti-hBid-BH3 Pab (Cat. #AP1307a) in mouse lung tissue lysates (35ug/lane). hBid-BH3(arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Western blot analysis of Bid (arrow) using rabbit polyclonal Bid Antibody (BH3) (Cat.#AP1307a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Bid gene.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Bid BH3 Domain Antibody (Cat.#AP1307a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:50~100
