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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunoprecipitation [2]
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Validation data
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- Product number
- GTX101323 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101323, RRID:AB_2036344
- Product name
- Bid antibody [N1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references A high-throughput pipeline for validation of antibodies.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 (GTX27909) 12% SDS PAGE GTX101323 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Bid antibody [N1C3] immunoprecipitates BID protein in IP experiments.IP samples: Jurkat whole cell extractA. 40 £gg Jurkat whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of BID protein by 4 £gg Bid antibody [N1C3] (GTX101323)5 % SDS-PAGEThe immunoprecipitated BID protein was detected by Bid antibody [N1C3] (GTX101323) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of Bid protein from Jurkat whole cell extracts using 5 £gg of Bid antibody [N1C3] (GTX101323) or Bid antibody [N1C3-2] (GTX110568).Western blot analysis was performed using Bid antibody [N1C3] (GTX101323).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
