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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [2]
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- Product number
- MAB860 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal BID Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse BID in Western blots. Detects full-length mouse BID and the 15 kDa carboxyl-terminal fragment (tBID) generated by cleavage with Caspase-8. The antibody also detects human BID. However, in Western blots of recombinant human and mouse BID, the antibody is most sensitive for mouse BID.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Impact of caspase-1/11, -3, -7, or IL-1β/IL-18 deficiency on rabies virus-induced macrophage cell death and onset of disease.
Sensing cytosolic RpsL by macrophages induces lysosomal cell death and termination of bacterial infection.
The PI3K regulatory subunits p55α and p50α regulate cell death in vivo.
BH3-only protein Bid is dispensable for seizure-induced neuronal death and the associated nuclear accumulation of apoptosis-inducing factor.
Activation of the extrinsic caspase pathway in cultured cortical neurons requires p53-mediated down-regulation of the X-linked inhibitor of apoptosis protein to induce apoptosis.
Proteolysis of HIP during apoptosis occurs within a region similar to the BID loop.
Kip E, Nazé F, Suin V, Vanden Berghe T, Francart A, Lamoral S, Vandenabeele P, Beyaert R, Van Gucht S, Kalai M
Cell death discovery 2017;3:17012
Cell death discovery 2017;3:17012
Sensing cytosolic RpsL by macrophages induces lysosomal cell death and termination of bacterial infection.
Zhu W, Tao L, Quick ML, Joyce JA, Qu JM, Luo ZQ
PLoS pathogens 2015 Mar;11(3):e1004704
PLoS pathogens 2015 Mar;11(3):e1004704
The PI3K regulatory subunits p55α and p50α regulate cell death in vivo.
Pensa S, Neoh K, Resemann HK, Kreuzaler PA, Abell K, Clarke NJ, Reinheckel T, Kahn CR, Watson CJ
Cell death and differentiation 2014 Sep;21(9):1442-50
Cell death and differentiation 2014 Sep;21(9):1442-50
BH3-only protein Bid is dispensable for seizure-induced neuronal death and the associated nuclear accumulation of apoptosis-inducing factor.
Engel T, Caballero-Caballero A, Schindler CK, Plesnila N, Strasser A, Prehn JH, Henshall DC
Journal of neurochemistry 2010 Oct;115(1):92-101
Journal of neurochemistry 2010 Oct;115(1):92-101
Activation of the extrinsic caspase pathway in cultured cortical neurons requires p53-mediated down-regulation of the X-linked inhibitor of apoptosis protein to induce apoptosis.
Tun C, Guo W, Nguyen H, Yun B, Libby RT, Morrison RS, Garden GA
Journal of neurochemistry 2007 Aug;102(4):1206-19
Journal of neurochemistry 2007 Aug;102(4):1206-19
Proteolysis of HIP during apoptosis occurs within a region similar to the BID loop.
Caruso JA, Reiners JJ Jr
Apoptosis : an international journal on programmed cell death 2006 Nov;11(11):1877-85
Apoptosis : an international journal on programmed cell death 2006 Nov;11(11):1877-85
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and Mouse BID by Western Blot. Detection of Human and Mouse BID by Western Blot.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and Mouse BID by Western Blot. Detection of Human and Mouse BID by Western Blot.
