Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF846 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal BID Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human BID in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse BID is observed.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Impact of p53 status on TRAIL-mediated apoptotic and non-apoptotic signaling in cancer cells.
Caspase-10: a molecular switch from cell-autonomous apoptosis to communal cell death in response to chemotherapeutic drug treatment.
Caspase-2-mediated cell death is required for deleting aneuploid cells.
TWEAK induces apoptosis through a death-signaling complex comprising receptor-interacting protein 1 (RIP1), Fas-associated death domain (FADD), and caspase-8.
Enhancement of stress-induced apoptosis in B-lineage cells by caspase-9 inhibitor.
Endoplasmic reticulum calcium pool depletion-induced apoptosis is coupled with activation of the death receptor 5 pathway.
Willms A, Schittek H, Rahn S, Sosna J, Mert U, Adam D, Trauzold A
PloS one 2019;14(4):e0214847
PloS one 2019;14(4):e0214847
Caspase-10: a molecular switch from cell-autonomous apoptosis to communal cell death in response to chemotherapeutic drug treatment.
Mohr A, Deedigan L, Jencz S, Mehrabadi Y, Houlden L, Albarenque SM, Zwacka RM
Cell death and differentiation 2018 Feb;25(2):340-352
Cell death and differentiation 2018 Feb;25(2):340-352
Caspase-2-mediated cell death is required for deleting aneuploid cells.
Dawar S, Lim Y, Puccini J, White M, Thomas P, Bouchier-Hayes L, Green DR, Dorstyn L, Kumar S
Oncogene 2017 May 11;36(19):2704-2714
Oncogene 2017 May 11;36(19):2704-2714
TWEAK induces apoptosis through a death-signaling complex comprising receptor-interacting protein 1 (RIP1), Fas-associated death domain (FADD), and caspase-8.
Ikner A, Ashkenazi A
The Journal of biological chemistry 2011 Jun 17;286(24):21546-54
The Journal of biological chemistry 2011 Jun 17;286(24):21546-54
Enhancement of stress-induced apoptosis in B-lineage cells by caspase-9 inhibitor.
Shah N, Asch RJ, Lysholm AS, Lebien TW
Blood 2004 Nov 1;104(9):2873-8
Blood 2004 Nov 1;104(9):2873-8
Endoplasmic reticulum calcium pool depletion-induced apoptosis is coupled with activation of the death receptor 5 pathway.
He Q, Lee DI, Rong R, Yu M, Luo X, Klein M, El-Deiry WS, Huang Y, Hussain A, Sheikh MS
Oncogene 2002 Apr 18;21(17):2623-33
Oncogene 2002 Apr 18;21(17):2623-33
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human BID by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 µg/mL anti-Fas Antibody for 6 hours and WS-1 human fetal skin fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human BID Polyclonal Antibody (Catalog # AF846), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for BID at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.