GTX110568

antibody from GeneTex

Targeting: BID

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX110568

Provider

GeneTex

Proper citation

GeneTex Cat#GTX110568, RRID:AB_1949744

Product name

Bid antibody [N1C3-2]

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

A high-throughput pipeline for validation of antibodies.

Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912

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Dandelion root extract affects colorectal cancer proliferation and survival through the activation of multiple death signalling pathways.

Ovadje P, Ammar S, Guerrero JA, Arnason JT, Pandey S
Oncotarget 2016 Nov 8;7(45):73080-73100

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Differentiation of human neuroblastoma cells toward the osteogenic lineage by mTOR inhibitor.

Carpentieri A, Cozzoli E, Scimeca M, Bonanno E, Sardanelli AM, Gambacurta A
Cell death & disease 2015 Nov 12;6(11):e1974

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Generation of homologous cell pairs using the oral lymphatic system.

Pu YF, Wang L, Wu HH, Bian H, Hong YY, Wang YX, Guo CB
International journal of clinical and experimental pathology 2014;7(4):1563-71

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Bid antibody [N1C3-2] detects Bid protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g A431 whole cell lysate/extract C. 30 ?g H1299 whole cell lysate/extract D. 30 ?g HeLa whole cell lysate/extract E. 30 ?g HepG2 whole cell lysate/extract F. 30 ?g Molt-4 whole cell lysate/extract G. 30 ?g Raji whole cell lysate/extract7.5 % SDS-PAGEBid antibody [N1C3-2] (GTX110568) dilution: 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 £gg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bid antibody [N1C3-2] (GTX110568) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Whole cell extract (30 £gg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bid antibody [N1C3-2] (GTX110568) diluted at 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Bid antibody [N1C3-2] detects BID protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37¢J for 30 min.Green: BID protein stained by Bid antibody [N1C3-2] (GTX110568) diluted at 1:500.Blue: Hoechst 33343 staining.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Bid antibody [N1C3-2] detects Bid protein at cytoplasm by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Bid protein stained by Bid antibody [N1C3-2] (GTX110568) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Hoechst 33342 staining.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Bid antibody [N1C3-2] immunoprecipitates BID protein in IP experiments.IP samples: Jurkat whole cell extractA. 40 ?g Jurkat whole cell extractB. Control with 4 ?g of preimmune Rabbit IgGC. Immunoprecipitation of BID protein by 4 ?g Bid antibody [N1C3-2] (GTX110568)5 % SDS-PAGEThe immunoprecipitated BID protein was detected by Bid antibody [N1C3-2] (GTX110568) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Validation comment

IP

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunoprecipitation of Bid protein from Jurkat whole cell extracts using 5 £gg of Bid antibody [N1C3-2] (GTX110568) or Bid antibody [N1C3] (GTX101323).Western blot analysis was performed using Bid antibody [N1C3-2] (GTX110568).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using Bid(GTX110568) antibody at 1:500 dilution.