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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- GTX25498 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX25498, RRID:AB_380561
- Product name
- Bid (p15-specific ) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Alternative Fas-mediated cell death pathway is dependent on the different cleavage patterns of procaspase-8.
Li Y, Qi L, Iwao A, Kihira K, Dida F, Song Z, Azuma E, Komada Y
Molecular and cellular biochemistry 2009 Nov;331(1-2):231-8
Molecular and cellular biochemistry 2009 Nov;331(1-2):231-8
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Western Blot Extracts of Jurkat cells with caspase-8 cleaved recombinant human BID (lane 1), or with caspase-8 cleaved recombinant mouse BID (lane 2) were resolved by SDS-PAGE on a 4-20% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with Bid (p15-specific ) antibody for two hours at room temperature in a 1% BSA-TBST buffer. The data show that the human BID (p15) antibody recognizes only the human 15 kDa BID fragment, demonstrating the specificity of the antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Extracts of Jurkat cells without added BID (lanes 1 and 2), with caspase-8 cleaved recombinant human BID (lane 3), or with caspase-8 cleaved recombinant mouse BID (lane 4) were resolved by SDS-PAGE on a 4-20% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with either a human BID full length antibody (lane 1) or the human BID (p15) cleavage site-specific antibody (lanes 2-4) for two hours at room temperature in a 1% BSA-TBST buffer. After washing, the membrane was incubated with goat F(ab)2 anti-rabbit IgG alkaline phosphatase and signals were detected. The data show that the human BID (p15) antibody recognizes only the human 15 kDa BID fragment, demonstrating the specificity of the antibody.
